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KMID : 1094720200250010009
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Biotechnology and Bioprocess Engineering
2020 Volume.25 No. 1 p.9 ~ p.15
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Effects of Time on Phenolics and in vitro Bioactivity in Autoclave Extraction of Graviola (Annona muricata) Leaf
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Choi Moon-Kyeung
Kang Yu-Ra
Zu Hyo-Don
Lim In-Sook
Jung Sung-Keun
Chang Yoon-Hyuk
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Abstract
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We investigated the effects of different autoclave extraction times (1-6 h) on the antioxidant, antidiabetic, and anti-inflammatory activities of phenolic compounds isolated from graviola (Annona muricata) leaves. In terms of yield and content of total polyphenols, total flavonoids, rutin, and kaempferol-3-O-rutinoside, the optimal extraction time was 4 h. At this time point, water extracts from graviola leaves (WEG) exhibited the highest DPPH radical scavenging activity, ferric reducing antioxidant power, and α-glucosidase inhibitory activity. The α-glucosidase inhibitory activity of WEG was significantly stronger than that of acarbose. Furthermore, WEG significantly suppressed lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells and inhibited UVB-induced activator protein-1 (AP-1)-dependent transcription in human keratinocytes. In conclusion, WEG can be used as functional food ingredients in the food industry.
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KEYWORD
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bioactive compounds, flavonoids, food ingredients, natural antioxidants, phenolics
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